The files required to reproduce these analyses are included in the R package directory on installation, and can be downloaded from GitHub.
If you have RStudio, you can
open the R Markdown file used to generate this document
(vignettes/Conduct-analyses.Rmd) to run the R scripts that
will copy all necessary files and begin analyses on your behalf. You
will need to specify some paths for automatic downloading:
# Directory in which to install MrBayes
BAYES_DIR <- "C:/Research/MrBayes"
# Directory in which to conduct parsimony analysis
HOME <- "C:/Research/iw" # Must not end in a trailing "/"
# GitHub remote
INST_ROOT <- "https://raw.githubusercontent.com/ms609/CongreveLamsdell2016/master/inst/"bayesgen.pl is a Perl script to execute analysis using
Markov models in MrBayes.
The script reads the datasets of Congreve and Lamsdell [1], appends a MrBayes block to the Nexus files, and executes a MrBayes run, saving the consensus trees and preparing them for analysis in R.
Before running the script:
MRBAYES_RELEASE <- "https://github.com/NBISweden/MrBayes/releases/download/v3.2.7/MrBayes-3.2.7-WIN.zip"
zipFile <- paste0(BAYES_DIR, "/MrBayes.zip")
download.file(MRBAYES_RELEASE, destfile = zipFile, method = "auto", mode = "wb")
unzip(zipFile,
files = "MrBayes-3.2.7-WIN/bin/mb.3.2.7-win64.exe",
exdir = BAYES_DIR,
junkpaths = TRUE
)
file.remove(zipFile)C:/Research/MrBayes/iw)tempFile <- tempfile(fileext = ".zip")
download.file("https://datadryad.org/bitstream/handle/10255/dryad.108351/S5%20-%20Character%20Weights%20Test%20NEXUS%20files.zip", tempFile)
unzip(
tempFile,
exdir = paste0(BAYES_DIR, "/iw"),
junkpaths = TRUE,
files = paste0("Weights tests/",
formatC(1:100, width = 3, flag = 0),
".txt.nex")
)
file.remove(tempFile)mrbayesblock.nex to the iw directory,
and bayesgen.pl and t2nex.pl to the root
MrBayes directory.C:/Research/MrBayes/) and path to extracted matrices
(default: C:/Research/MrBayes/iw)download.file(paste0(INST_ROOT, "analysis-bayesian/mrbayesblock.nex"),
paste0(BAYES_DIR, "/iw/mrbayesblock.nex"))
bayesGenPath <- paste0(BAYES_DIR, "/bayesgen.pl")
download.file(paste0(INST_ROOT, "analysis-bayesian/bayesgen.pl"), bayesGenPath)
bayesGen <- readLines(bayesGenPath)
bayesGen[5] <- paste0('$dir = "', BAYES_DIR, '/iw";')
bayesGen[6] <- paste0('$bayes_dir = "', BAYES_DIR, '";')
writeLines(bayesGen, bayesGenPath)
t2nexPath <- paste0(BAYES_DIR, "/t2nex.pl")
download.file(paste0(INST_ROOT, "analysis-bayesian/t2nex.pl"), t2nexPath)
t2nex <- readLines(t2nexPath)
t2nex[2] <- paste0('$dir = "', BAYES_DIR, '/iw";')
writeLines(t2nex, t2nexPath)Perform the analyses by executing bayesgen.pl. (Once
Perl is installed, you can just double-click the file.)
Once the analyses are complete, copy all files ending
.run#.nex to C:/Research/iw/MrBayes.
mptgen.pl is a Perl script to generate most parsimonious
trees by parsimony search in TNT.
The script generates TNT scripts to perform parsimony analysis on each of the Congreve and Lamsdell datasets, under equal and implied weights, with and without suboptimal trees. It then executes these scripts and converts the output into a format suitable for analysis in R.
Before running the script, you’ll need an installation of Perl. Strawberry Perl works on MS Windows.
Then:
C:/Research/iw) with
subdirectories entitled Matrices, and Trees.
Then, within the new Trees directory, create the further
subdirectories eq, k1, k2,
k3, k5 and kX.sapply(paste0(HOME, "/", c("", "Matrices", "Trees")), dir.create)
sapply(paste0(HOME, "/Trees/", c("eq", "k1", "k2", "k3", "k5", "kX")), dir.create)zipFile <- paste0(HOME, "/TNT.ZIP")
# This is the Windows path; use the appropriate path for your operating system
download.file("http://www.lillo.org.ar/phylogeny/tnt/ZIPCHTNT.ZIP",
destfile=zipFile, method="auto", mode="wb")
unzip(zipFile, "tnt.exe", exdir=HOME)
file.remove(zipFile)mptgen.pl and (optionally) tnt2nex.pl
into this root directory, updating each file so its variable $dir
corresponds to the appropriate path.tnt2nex.pl translates TNT output into NEXUS format and may
be useful if you wish to perform further analysis of TNT output. This
will be performed automatically if you uncomment the final line of
mptgen.pl.tnt2nexPath <- paste0(HOME, "/tnt2nex.pl")
mptgenPath <- paste0(HOME, "/mptgen.pl")
download.file(paste0(INST_ROOT, "analysis-parsimony/tnt2nex.pl"), tnt2nexPath)
tnt2nex <- readLines(tnt2nexPath)
tnt2nex[3] <- paste0('$dir = "', HOME, '/Trees";')
writeLines(tnt2nex, tnt2nexPath)
download.file(paste0(INST_ROOT, "analysis-parsimony/mptgen.pl"), mptgenPath)
mptgen <- readLines(mptgenPath)
mptgen[3] <- paste0('$dir = "', HOME, '";')
writeLines(mptgen, mptgenPath)tnt_template.run into the root
directory.download.file(paste0(INST_ROOT, "analysis-parsimony/tnt_template.run"),
paste0(HOME, "/tnt_template.run"))Matrices.tempFile <- tempfile(fileext=".zip")
download.file("https://datadryad.org/bitstream/handle/10255/dryad.101095/S1%20-%20TNT%20files.zip", tempFile)
unzip(tempFile, exdir=paste0(HOME, "/Matrices"))mptgen.pl. (Once Perl
is installed, you can just double-click the file.)Once these analyses have generated the necessary data, these can be analysed using the scripts in [https://github.com/ms609/CongreveLamsdell2016/blob/master/data-raw/GenerateData.Rmd]. The results of these analyses are available in the R data objects; to view them, install the package in R and view the help files.